Dr. June H. Middleton
Courses Taught at FDU
Introduction to Microbes
Biological Science II. Introduction to Molecules, Cells and Genes
In my lab we monitor the prevalence and frequency of specific antibiotic resistance and human virulence factor expression in environmental microorganisms. We are especially interested tracking expression in the fecal indicator organisms E. coli and Enterococcus spp. The emerging nosocomial pathogens of man, E. faecalis and E. faecium, are of special interest. Our studies will provide insights about environmental transmission of pathogenicity determinants in surface waters and by wildfowl. A new area of research involves examining how environmental microbes respond to the presence of low levels of triclosan, a commonly used antibacterial, especially with respect to changes in antibiotic resistance patterns.
Ph.D., U. North Carolina (Chapel Hill), Microbiology and Immunology
B.A., Douglass College (Rutgers), Bacteriology/Chemistry
Wentworth, D.K., and J.H. Middleton. 2014. Technology use and academic performance. Computers & Education 78:306-311.
Middleton, J.H. and D. Chamberlain*. 2013. Prevalence, Antibiotic Resistance and Virulence Factor Expression of Enterococcus faecalis and Enterococcus faecium Isolates from the Upper Passaic River, Morris County, NJ. Bulletin of the New Jersey Academy of Science. 58(1):1-10.
Middleton, J.H. and J.D. Salierno. 2013. Antibiotic resistance in triclosan tolerant fecal coliforms isolated from surface waters near wastewater treatment plant outflows (Morris County, NJ, USA). Ecotoxicology and Environmental Safety 88:79-88.
Middleton, J. H. 2007. Evaluating Bacteriocin Production by Environmental Enterococci: An Inquiry Based Activity in Bacterial Antagonism. The American Biology Teacher 69(5):364-369.
Middleton, J. H. and A. Ambrose*. 2005. Enumeration and Antibiotic Resistance Patterns of Fecal Indicator Organisms Isolated from Migratory Canada Geese (Branta canadensis).Journal of Wildlife Diseases 41(2).
Middleton, June H. and Danielle Duong*. 2003. Antibiotic Resistance Patterns In Fecal Indicator Organisms Isolated from Resident Canada Geese In Morris County. The Bulletinof the New Jersey Academy of Science 48(2):1-7.
Recent Conference Presentations
Middleton, J.H. Prevalence of Antibiotic Resistance in Fecal E.coli of Migratory Canada Geese (Branta canadensis) Parallels Antibiotic Usage in Animal Agriculture: a Longitudinal Study 2002 -2011. 113th General Meeting, American Society for Microbiology. Denver, CO. May 18-21, 2013.
Middleton, J.H., Myers, E.M., Gardner, M.K*., Graciano, T.R.*, and Lettenberger, K*. Use of a Simplified C. elegans Assay to Detect Expressed Virulence Factors in Environmental Enterococcus faecalis Isolates. 112th General Meeting, American Society for Microbiology. San Francisco, CA. June 18, 2012.
Myers, E. R and J.H. Middleton. Using C. elegans as a model to assay bacterial pathogenesis in an undergraduate research survey course. 18th Annual International C. elegans Meeting. UCLA. June 2011. (Presenter: E. Myers)
Middleton, J.H. and J. Salierno. Triclosan Resistance in Thermotolerant Fecal Indicator Organisms Isolated from WWTP Outflows. 111th General Meeting, American Society for Microbiology. New Orleans, LA. May 23, 2011.
Sheth, M.* and J. Middleton. Effect of Triclosan Concentration and Exposure Time of Specific Antibiotic Resistance in E. coli Strain K-12 and ATCC 25922 in TSB or River Water.110th General Meeting, American Society for Microbiology. San Diego, CA. May 22, 2010.
Middleton, J. 2009. Community Bacteriocin Expression in Enterococci from Resident Geese. 109th General Meeting of the American Society for Microbiology. Philadelphia, PA.
Middleton, J, and D. Chamberlain. 2008. Evaluation of E. Faecalis and E. Faecium Populations in the Upper Passaic River, Morris County, NJ. Third Passaic River Symposium. Montclair State University, Montclair, NJ.
Chamberlain, D. and J. Middleton. June 2008. Evaluation of E. faecalis and E. faecium Populations in the Upper Passaic River, Morris County, NJ. 108th General Meeting of the American Society for Microbiology. Boston, MA.
Chamberlain, D.* and J. Middleton. October 2007. Evaluation of E. faecalis and E. faecium. 40th Annual meeting of the Metropolitan Association of College and University Biologists (MACUB), St. John’s U., Queens, NY. Won prize for best poster.
Middleton, J. and D. Twitchell*. May 2007. Changes in Enterococcal Populations along the Upper Passaic River in Morris County , NJ. 107th General Meeting of the American Society for Microbiology. Toronto, ONT.
Chamberlain, D.* and J. Middleton. April 2007. Antibiotic Resistance in Escherichia Coli Isolated from Migratory Canada Geese - A Five Year Study. New Jersey Academy of Science Annual Meeting. Kean U.
Horvath, L.M.* and J. H. Middleton. May 2006. Detection of Expressed Virulence Factors in Enterococcus faecalis and Enterococcus faecium Isolated from Canada Goose Feces. 106th General Meeting of American Society for Microbiology, Orlando, FL.
Twitchell*, David F. and June H. Middleton. April 2006. Expression of Virulence Factors In Enterococcus Faecalis And Enterococcus Faecium Isolated From Migratory Canada Geese. American Society for Microbiology (ASM) Mini-Meeting, Rutgers, New Brunswick, NJ.
* indicates undergraduate research student
American Society for Microbiology
Theobald Smith Society – National Councilor
Metropolitan Association of College and University Biologists
New Jersey Academy of Science
FDU Faculty Grant-in-Aid (2013-14)
Merck/AAAS Undergraduate Science Research Program award (2005)
SENCER – NSF sub-grant
Phone: (973) 443-8772
Email: junem @ fdu.edu
Office: Science 29